Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. Optimized settings for running LAMP experiments on isothermal instruments such as the Axxin T8-ISO and T16-ISO can be found here. Instrumentation for LAMP typically requires consistent heating to the desired reaction temperature and, where needed, real-time fluorescence for quantitative measurements. Real-time fluorescence detection using intercalators or probes, lateral flow and agarose gel detection are all directly compatible with LAMP reactions. These products are not typically appropriate for downstream manipulation, but target amplification is so extensive that numerous modes of detection are possible. DNA products are very long (>20 kb) and formed from numerous repeats of the short (80–250 bp) target sequence, connected with single-stranded loop regions in long concatamers. A strand-displacing DNA polymerase initiates synthesis and 2 specially designed primers form “loop” structures to facilitate subsequent rounds of amplification through extension on the loops and additional annealing of primers. Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA for a highly specific amplification reaction.
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